Study on the Correlation between the Structure and Toxicity of Amines, Mercaptans and Halohydrocarbons

The aim of the present work was to understand the structure of amines, mercaptans and halohydrocarbons and their cell toxicity effect on rat liver BRL-3A cells. BRL-3A cells were seeded into each well of 96-well plates and treated with amines, mercaptans and halohydrocarbons (total 18 kinds) in DMEM culture medium containing 10% fetal calf serum. 24h later, the growth viability in vitro of BRL3A cells was measured using the MTT assay. The results show that the cytotoxic of propane diamine> ethidene diamine> n-butylamine> n-propylamine> ethylamine, butyl mercaptan> propanethiol> disulfide propane> dithioglycol, n-Butyl iodide> n-Propyl iodide> iodoethane, propylene bromide> 1, 2-dibromoethane> ethyl bromide, ethylene dichloride> butyl chloride> chloropropane. We can conclude that among amines, when the length of carbon chain is fixed, the more amidogen, the stronger the toxicity; when the number of hydroxyl group is fixed, the longer carbon chain, the stronger the toxicity. Among mercaptans, when the length of carbon chain is the same, the more hydrosulphonyl, the weaker the toxicity; when the number of hydrosulphonyl is the same, the longer carbon chain, the weaker the toxicity. Among halohydrocarbons, when the length of carbon chain is fixed, the more halogen, the stronger the toxicity; when the number of halogen is fixed, the longer carbon chain, the stronger the toxicity. Under the same conditions, the toxicity of mercaptans is greater than amines and halohydrocarbons.