IDEAS home Printed from https://ideas.repec.org/a/ajp/edwast/v9y2025i1p1010-1018id4305.html
   My bibliography  Save this article

Evaluation of the sensitivity and specificity of monoclonal antibodies for detecting the thioredoxin antigen in hepatocellular carcinoma patients

Author

Listed:
  • Duong Quang Huy
  • Ho Truong Giang
  • Nguyen Thi Diem
  • Vo Thi Bich Thuy

Abstract

The objective of this study was to evaluate the sensitivity and specificity of the monoclonal antibody of thioredoxin (mAb-TXN) for detecting the thioredoxin (TXN) antigen in serum samples from patients with hepatocellular carcinoma (HCC). To achieve this, the mAb-TXN, generated from hybridoma cells, was utilized to optimize the conditions for an indirect ELISA immunoassay. The sensitivity, specificity, positive predictive value, and negative predictive value of the assay were evaluated on the basis of stored patient medical records. The results showed that indirect ELISA using mAb-TXN exhibited a sensitivity of 93.61%, a specificity of 96%, a positive predictive value of 97.77%, and a negative predictive value of 88%. In conclusion, mAb-TXN is a valuable tool for detecting TXN antigens in serum samples from HCC patients, offering high sensitivity and specificity for clinical application.

Suggested Citation

  • Duong Quang Huy & Ho Truong Giang & Nguyen Thi Diem & Vo Thi Bich Thuy, 2025. "Evaluation of the sensitivity and specificity of monoclonal antibodies for detecting the thioredoxin antigen in hepatocellular carcinoma patients," Edelweiss Applied Science and Technology, Learning Gate, vol. 9(1), pages 1010-1018.
    • Handle: RePEc:ajp:edwast:v:9:y:2025:i:1:p:1010-1018:id:4305
    as

    Download full text from publisher

    File URL: https://learning-gate.com/index.php/2576-8484/article/view/4305/1664
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ajp:edwast:v:9:y:2025:i:1:p:1010-1018:id:4305. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Melissa Fernandes (email available below). General contact details of provider: https://learning-gate.com/index.php/2576-8484/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.