IDEAS home Printed from https://ideas.repec.org/a/ajp/edwast/v8y2024i5p2157-2166id1967.html
   My bibliography  Save this article

Characteristic of neurogenic secretome under hypoxia: Organoleptic, IL-6 and IL-10 profile

Author

Listed:
  • Bagus Wibowo Soetojo
  • Teddy Heri Wardhana
  • Heri Suroto

Abstract

Understanding the physical characteristics and IL-6/IL-10 cytokine levels of neurogenic secretome is crucial for evaluating the effectiveness of stem cell therapy in nerve injury treatment. This study utilized an in vitro experimental design to analyze the organoleptic properties, pH levels, moisture content, and structural features of neurogenic secretome under hypoxia. Electron microscopy was used to examine structural details, while ELISA tests were performed to measure IL-6 and IL-10 cytokine concentrations. The control group exhibited a pale pink color in both liquid and freeze-dried forms, while the neurogenic secretome appeared light orange in liquid form and yellow when freeze-dried. Both samples had a neutral pH of 7.00. The control group showed a higher moisture content (5.510%) compared to the neurogenic secretome (5.099%). Electron microscopy revealed more pores in the neurogenic secretome than in the control (p=0.237). Additionally, cytokine analysis showed significantly lower levels of IL-6 and IL-10 in the neurogenic secretome compared to the control group (p<0.0001). The neurogenic secretome exhibited distinct physical characteristics, including more pores and lower cytokine levels, which may enhance cell proliferation and adhesion, potentially improving its effectiveness in stem cell therapy for nerve injuries. The findings suggest that neurogenic secretome, with its unique properties, could offer advantages in therapeutic applications involving nerve regeneration.

Suggested Citation

  • Bagus Wibowo Soetojo & Teddy Heri Wardhana & Heri Suroto, 2024. "Characteristic of neurogenic secretome under hypoxia: Organoleptic, IL-6 and IL-10 profile," Edelweiss Applied Science and Technology, Learning Gate, vol. 8(5), pages 2157-2166.
    • Handle: RePEc:ajp:edwast:v:8:y:2024:i:5:p:2157-2166:id:1967
    as

    Download full text from publisher

    File URL: https://learning-gate.com/index.php/2576-8484/article/view/1967/743
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ajp:edwast:v:8:y:2024:i:5:p:2157-2166:id:1967. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Melissa Fernandes (email available below). General contact details of provider: https://learning-gate.com/index.php/2576-8484/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.