IDEAS home Printed from https://ideas.repec.org/a/ags/ijarit/317024.html
   My bibliography  Save this article

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Author

Listed:
  • MRI Mallik
  • MM Sikder
  • MK Hossain
  • MB Billah
  • N Alam

Abstract

Fruit rot disease of sweet pepper is one of the main fungal diseases causing huge economic losses to the grower. An experiment was conducted to find out the fungal pathogen associated with fruit rot disease of sweet pepper, obtained from experimental fields of Jahangirnagar University, Bangladesh. Fruit rot disease-causing fungus was isolated from infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well as molecular characterization based on internal transcribe spacer (ITS) region of the fungus. ITS sequence of our studied fungus MH368146.1 was genetically 99-100% similar to sequences of Fusarium solani in NCBI database. Typical fruit rot symptoms were reproduced by artificial inoculations of the isolated fungus. The mycelial growth of this fungus was evaluated on ten different solid culture media i.e., Potato Dextrose Agar, Yeast Extract Agar, Honey Peptone Agar, Hansen’s Medium, Sabouraud’s Glucose Agar, Kauffman’s Agar, Potato Sucrose Agar, Richard’s Agar and Carrot Agar. Fungus grew well on all tested solid culture media. Several bio-control agents and two commercial fungicides were evaluated against isolated fungus under in vitro condition, in which the highest percent inhibition of radial growth of the fungus was determined as 64.75% due to Trichoderma reesei isolate 2, and 60.63% by Tilt 250 EC (500 ppm) at 7 days post-incubation. Therefore, T. reesei was found as the most suitable to control the growth of F. solani under laboratory conditions. However, further pot and field trials needed to be confirmed the bio-control potential of it.

Suggested Citation

  • MRI Mallik & MM Sikder & MK Hossain & MB Billah & N Alam, 2021. "Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper," International Journal of Agricultural Research, Innovation and Technology (IJARIT), IJARIT Research Foundation, vol. 11(2), December.
  • Handle: RePEc:ags:ijarit:317024
    DOI: 10.22004/ag.econ.317024
    as

    Download full text from publisher

    File URL: https://ageconsearch.umn.edu/record/317024/files/15%29%20IJARIT%200355.pdf
    Download Restriction: no

    File URL: https://libkey.io/10.22004/ag.econ.317024?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sikder, M.M. & Ahmmed, M.S. & Sultana, A. & Alam, N., 2020. "First report on black spot disease of Phyllanthus emblica L. fruits caused by Thielaviopsis paradoxa in Bangladesh," International Journal of Agricultural Research, Innovation and Technology (IJARIT), IJARIT Research Foundation, vol. 10(2), December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. MB Billah & MM Sikder & MRI Mallik & MK Hossain & N Alam, 2021. "Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures," International Journal of Agricultural Research, Innovation and Technology (IJARIT), IJARIT Research Foundation, vol. 11(2), December.

    More about this item

    Keywords

    Crop Production/Industries;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ags:ijarit:317024. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: AgEcon Search (email available below). General contact details of provider: https://ijarit.webs.com/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.