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DEVELOPMENT OF EFFICIENT CALLUS INITIATION OF MALTA (Citrus sinensis) THROUGH TISSUE CULTURE

Author

Listed:
  • Azim, Fazle
  • Rahmna, M.M.
  • Prodham, Shamsul H.
  • Sikdar, Saif U.
  • Zobayer, Nayem
  • Ashrafuzzaman, M.

Abstract

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.

Suggested Citation

  • Azim, Fazle & Rahmna, M.M. & Prodham, Shamsul H. & Sikdar, Saif U. & Zobayer, Nayem & Ashrafuzzaman, M., 2011. "DEVELOPMENT OF EFFICIENT CALLUS INITIATION OF MALTA (Citrus sinensis) THROUGH TISSUE CULTURE," International Journal of Agricultural Research, Innovation and Technology (IJARIT), IJARIT Research Foundation, vol. 1(01-2), December.
    • Handle: RePEc:ags:ijarit:305268
    DOI: 10.22004/ag.econ.305268
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    Crop Production/Industries;

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