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Vitamin C is Required To Allow Sustained IL-1β-Induced Suppression of The Pyrophosphate Regulators ANKH and ENPP1 in Bovine Nucleus Pulposus Cells

Author

Listed:
  • Robert J Frawley
  • AgataKrzyzanowska MS
  • Matthew E. Cunningham

    (Hospital for Special Surgery, USA
    Weill Cornell Graduate School of Medical Sciences, USA)

Abstract

The extracellular concentration of pyrophosphate (PPi) is increased by the activity of proteins like ectonucleotidepyrophosphatase/phsophodiesterase (ENPP1) and progressive alkalosis protein homolog (ANKH), whereas the concentration of PPi is decreased by tissue non-specific alkaline phosphatase (TNAP) through hydrolysis. Since PPi prevents calcium-phosphate deposition, ANKH and ENPP1 inhibit tissue mineralization and TNAP enhances mineralization. The presence of the inflammatory cytokine Interleukin-1 (IL-1)β alters expression of important regulatory proteins in many cells. The objective was to determine the effect of IL-1β on the gene expression of ANKH and ENPP1 in bovine Nucleus pulposus (bNP) cells, and the importance of vitamin C.A dose-dependent increase in calcium staining of TNAP-transduced bNP cell cultures was observed with alizarin red, indicating increased calcium deposition in the matrix at 3 and 7 days when treated with IL-1β.

Suggested Citation

  • Robert J Frawley & AgataKrzyzanowska MS & Matthew E. Cunningham, 2017. "Vitamin C is Required To Allow Sustained IL-1β-Induced Suppression of The Pyrophosphate Regulators ANKH and ENPP1 in Bovine Nucleus Pulposus Cells," Orthopedics and Rheumatology Open Access Journals, Juniper Publishers Inc., vol. 5(4), pages 95-100, April.
    • Handle: RePEc:adp:joroaj:v:5:y:2017:i:4:p:95-100
    DOI: 10.19080/OROAJ.2017.05.555674
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