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Limit of Blank (LOB), Limit of Detection (LOD), and Limit of Quantification (LOQ)

Author

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  • Mohamad Taleuzzaman

    (Assistant Professor, Pharmaceutical Chemistry, Glocal School of Pharmacy, Glocal University, India)

Abstract

For any analytical method development and validation, it is require developing arobust, accurate, precise, and linear method which is the heart for the development activities for drug product and drug substances. It is vital importance to initiate through a systematic process that the analytical method under question is acceptable for its intended purpose. Determining the limit of what and require for the quantitative determination is according of regulatory authorities globally. Limit of detection (LOD) and limit of quantitation (LOQ) can be found in the International Conference on Harmonization’s (ICH) Q2 Validation of Analytical Procedures. LOD and LOQ are parameters employ to explain the smallest concentration of an analyte that can be reliably measured by an analytical procedure. The LOQ is lowest concentration that quantitatively measured suitably with accuracy and precision while the LOD is the concentration that can be detected. The Most typical practice for determining the LOD /LOQ is to determine ratio of signal to noise. If the ratio is 3:1 it is LOD and if it is 10:1 than it is LOQ.

Suggested Citation

  • Mohamad Taleuzzaman, 2018. "Limit of Blank (LOB), Limit of Detection (LOD), and Limit of Quantification (LOQ)," Organic & Medicinal Chemistry International Journal, Juniper Publishers Inc., vol. 7(5), pages 127-131, August.
    • Handle: RePEc:adp:jomcij:v:7:y:2018:i:5:p:127-131
    DOI: 10.19080/OMCIJ.2018.07.555722
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