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Development and Validation of an RP-HPLC Method for Estimation of Secnidazole and Its Degradation Products in Tablets

Author

Listed:
  • Ali Gamal Ahmed Al-Kaf
  • Ali Abdo Saif Ahmed Algaradi

    (Department of medicinal and analytical chemistry, Sana’a University, Yemen)

  • EL-Rashed. Ahmed Gad kariem

    (Department of pharmaceutical chemistry, Khartoum University, Sudan)

  • Talal Alssmani

    (Department of pharmaceutical chemistry, Omdurman Islamic University, Sudan)

Abstract

A simple, precise, specific, sensitive and accurate stability indicating RP-HPLC method for determination of secnidazole and its degradation products in tablets. The separation was performed on teknokroma, tracer excel C18 column (25cm x 0.46cm.5μm) using mobile phase consisting of water: methanol: acetonitrile in ratio (73: 17: 10). A flow rate was set at 1mL/min; the detection wavelength was set at 228nm. The calibration curves were found to be linear in the concentration range of 50-150/mL (r2 =0.9997) and 0.25-7.5μg/mL (r2 =0.998) at assay level and low-level of secnidazole. The percentage recoveries of secnidazole were 100.76-101.66% and 97.87-102% at assay and low-level, respectively at 95% confidence limit. The intraday precision was 0.553% and 1.35% at assay level and low-level, respectively. The intermediate precision was 0.56% and 3.10% at assay level and low-level, respectively.

Suggested Citation

  • Ali Gamal Ahmed Al-Kaf & Ali Abdo Saif Ahmed Algaradi & EL-Rashed. Ahmed Gad kariem & Talal Alssmani, 2016. "Development and Validation of an RP-HPLC Method for Estimation of Secnidazole and Its Degradation Products in Tablets," Global Journal of Pharmacy & Pharmaceutical Sciences, Juniper Publishers Inc., vol. 1(1), pages 9-17, March.
    • Handle: RePEc:adp:jgjpps:v:1:y:2016:i:1:p:9-17
    DOI: 10.19080/GJPPS.2016.01.555552
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