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GC-MS and HPLC Analysis of Chlorophytum Borivilianum (Safed Musli) A Plant from Ayurveda- Herbal Viagra

Author

Listed:
  • Ruchi Vyas
  • Garima Sharma
  • Adya Chaturvedi
  • Devki
  • Rashmi Sisodia

    (Department of Zoology, University of Rajasthan, Jaipur, India)

Abstract

The objective of this study was to determine the photochemical content of Chlorophytum borivilianum by GC-MS analysis and HPLC. Chlorophytum borivilianum is an endangered medicinal plant having several phyto constituents, which makes it suitable for many therapeutic applications. GC-MS analysis was carried out on a Shimadzu GCMSQP2010 Ultra system. Methanol is used as a solvent. The injector temperature was 280°C. The samples were injected in the split mode with split ratio 1 to 60. Injection volume was 1 μl. A capillary column Rtx-5MS (5% Diphenyl-95% Dimethyl Polysiloxane), 30 m x 0.25mm x 0.25 μm, was used. The carrier gas was helium with a constant flow of 1.00 ml/min.GC-MS analysis of drug led to the identification of 9 compounds viz., 5-methylhex-2-yl pentadecyl ester (23.69%), 9,10-Anthracenedione (19.02%), Phthalic acid (18.63%) etc. High-performance liquid chromatography was conducted to enumerate active ingredient in the plant extracts. These analyses help us to understand the importance of various phyto chemicals and also open up new ways to exploit a particular plant for various therapeutic and restorative purposes.

Suggested Citation

  • Ruchi Vyas & Garima Sharma & Adya Chaturvedi & Devki & Rashmi Sisodia, 2020. "GC-MS and HPLC Analysis of Chlorophytum Borivilianum (Safed Musli) A Plant from Ayurveda- Herbal Viagra," Global Journal of Reproductive Medicine, Juniper Publishers Inc., vol. 7(5), pages 101-1062:7, June.
    • Handle: RePEc:adp:jgjorm:v:7:y:2020:i:5:p:101-106
    DOI: 10.19080/GJORM.2020.07.555723
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