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Optimization of Sperm Culture Conditions for Human Assisted Reproductive Technologies

Author

Listed:
  • Carol L Curchoe

    (San Diego Fertility Center, San Diego, USA)

  • Charles L Bormann

    (Harvard Medical School, Obstetrics/Gynecology/Reproductive Endocrinology and Infertility, USA)

Abstract

Optimizing sperm performance can be a differentiating factor in clinical pregnancy rates for assisted reproductive technology (ART) laboratories. Minimizing cellular stress during routine sperm processing by maintaining a precise and stable environment is of great importance in the field. In commercial media, pH of the external cellular culture environment (pHe) is typically maintained at 7.2-7.4 by various buffering reagents, while the internal pH of the cell (pHi) depends on available lactate and amino acids. Combining buffers in solution has been previously shown to be valuable for stabilizing internal and external pH for various biological systems. We hypothesized that a dual-buffer culture medium might improve sperm performance for ART. Here we demonstrate superior performance of a commercially available dual-buffer solution of HEPES and MOPS: Multipurpose Handling Medium (MHM, Irvine Scientific). Significantly better performance, assessed at 8 and 48 hours, and measured as: sperm viability, total motility, and rapid forward progression, was observed for MHM over single buffer controls.

Suggested Citation

  • Carol L Curchoe & Charles L Bormann, 2018. "Optimization of Sperm Culture Conditions for Human Assisted Reproductive Technologies," Global Journal of Reproductive Medicine, Juniper Publishers Inc., vol. 3(5), pages 98-102, March.
    • Handle: RePEc:adp:jgjorm:v:3:y:2018:i:5:p:98-102
    DOI: 10.19080/GJORM.2018.03.555625
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