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Generation of Vascular Networks from Adipocytes In Vitro

Author

Listed:
  • Ana M Blázquez Medela
  • Ashley Penton

    (Division of Cardiology, David Geffen School of Medicine at UCLA, USA)

  • Kristina I Bostrom
  • Arman Saparov
  • Medet Jumabay

    (Department of Medicine, Nazarbayev University School of Medicine, Kazakhstans)

Abstract

Multipotent cells derived from white mature adipocytes, referred to as dedifferentiated fat (DFAT) cells have the capacity differentiate into endothelial cells. The objective of this study was to modify the isolation method for DFAT cells in order to optimize the endothelial lineage potential. The adipocytes were preincubated for 24 hours, washed, and then incubated for 5 days to allow the generated DFAT cells to remain in proximity to the adipocytes while the cells aggregated into cell clusters. The DFAT cells rapidly differentiated into adipocytes after which endothelial-like cells (ECs) emerged and formed tube-like structure closely associated with the newly differentiated adipocytes. The lipid-filled cells then gradually disappeared whereas the network of tube structure expanded over the course of 3 weeks. ECs accounted for 35-45% of the cells derived from the DFAT cells, as assessed by qPCR, immunofluorescence and fluorescence-activated cell sorting. The DFAT cell-derived ECs could also be further enriched by magnetic sorting, thereby serving as a mouse cell line for further research.

Suggested Citation

  • Ana M Blázquez Medela & Ashley Penton & Kristina I Bostrom & Arman Saparov & Medet Jumabay, 2019. "Generation of Vascular Networks from Adipocytes In Vitro," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 6(2), pages 40-45, August.
    • Handle: RePEc:adp:ijcsmb:v:6:y:2019:i:2:p:40-45
    DOI: 10.19080/IJCSMB.2019.06.555684
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