IDEAS home Printed from https://ideas.repec.org/a/adp/ijcsmb/v5y2018i1p49-56.html
   My bibliography  Save this article

Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines

Author

Listed:
  • Anamika Thakur
  • Sanju Kumari
  • Utkarshini Sharma
  • Rohit Krishna
  • Kanak Sinha
  • Santosh Kumar

    (Department of Botany, B R A Bihar University, India)

Abstract

Proteases are highly required where proteins need some sort of alteration either structurally or functionally. They are widely used in household laundry, paper and leather industries as well as in several pharmaceutical compounds. Thus, the present study was confined to screen more than fifty isolates of microbes from the rhizospheres of fruit orchard for proteolytic activity on skimmed milk agar medium. Isolates SK701 and SK709 were found producing halo regions around the streaked mycelia. However, only isolate SK709 displayed maximum 64.6 U/ml protease activities in starch casein broth after 96 hours of culture at optimum conditions of 35°C and pH 7.6. It was identified as a gram-negative bacterium on the basis of staining, morphological, cultural, biochemical and physiological characteristics.

Suggested Citation

  • Anamika Thakur & Sanju Kumari & Utkarshini Sharma & Rohit Krishna & Kanak Sinha & Santosh Kumar, 2018. "Molecular Characterization of Protease Producing Stenotrophomonas and Their Cytotoxic Effects on Cancer Cell Lines," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 5(1), pages 49-56, October.
    • Handle: RePEc:adp:ijcsmb:v:5:y:2018:i:1:p:49-56
    DOI: 10.19080/IJCSMB.2018.04.555660
    as

    Download full text from publisher

    File URL: https://juniperpublishers.com/ijcsmb/pdf/IJCSMB.MS.ID.555660.pdf
    Download Restriction: no
    File URL: https://juniperpublishers.com/ijcsmb/IJCSMB.MS.ID.555660.php
    Download Restriction: no
    File URL: https://libkey.io/10.19080/IJCSMB.2018.04.555660?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adp:ijcsmb:v:5:y:2018:i:1:p:49-56. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Robert Thomas (email available below). General contact details of provider: .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.