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Investigation of the Mitochondrial Haplogroups in A Selective Population of Isfahan Province

Author

Listed:
  • Elham Iranpour
  • Elmira Sanjari

    (Nour Danesh Institute of Higher Education, Iran)

  • Hasan Bahmani
  • Asmaolhosna Amini
  • Hasan Abbasi

    (National Institute of Genetic Engineering and Biotechnology, Iran)

  • Mahmoud Tavallaie

    (Nour Human Genetics Research Institute, Iran)

  • Massoud Houshmand

    (Environment Department, Isfahan University, Iran)

Abstract

In order to investigate the mitochondrial haplogroups in a selective population of Isfahan province, 96 unrelated men in Isfahan province with at least two generations in this province were token blood. Then DNA was extracted and quality assessed. 25 coding SNPs defining the major haplo groups that occur in Africa, Western Eurasia and Eastern Eurasia were selected and combined into two multiplex genotyping assays. Each one consisting of a PCR step and a SNaPShot step. Then for support of our detective haplo groups by SNaPShot system, D-Loop region was sequenced in some samples. : In this province Western Eurasian haplo groups were predominant. Haplo group U and H (22%, 21%) and then T, J and U8b (11%, 10% and 7%) had the most frequency. Eastern Eurasian haplo groups (C, D, R9, R11, M and N) were present at a lower frequency and a North African haplo group (M1) at frequency of 1%

Suggested Citation

  • Elham Iranpour & Elmira Sanjari & Hasan Bahmani & Asmaolhosna Amini & Hasan Abbasi & Mahmoud Tavallaie & Massoud Houshmand, 2018. "Investigation of the Mitochondrial Haplogroups in A Selective Population of Isfahan Province," International Journal of Cell Science & Molecular Biology, Juniper Publishers Inc., vol. 4(3), pages 59-68, April.
    • Handle: RePEc:adp:ijcsmb:v:4:y:2018:i:3:p:59-68
    DOI: 10.19080/IJCSMB.2018.03.555640
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