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Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Author

Listed:
  • Warta Dewi
  • Indrati
  • Inne Suherna Sasmita

Abstract

Objective: to test the extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.) As dye substitution in Gram staining technique. Material and Methods: Made Preparations from the suspension Escherichia coli ATCC 25922 and Streptococcus sanguine ATCC 10556 counted 30 preparation, and then done Gram staining process to this preparation. 1 preparation using counterstain basic fuchsin as a control and 29 other preparations using ethyl acetate extract of Red Fruit as a counterstain, the result of staining compared to using standard degradation of RHS color chart. Result: From 29 sample preparations used the counterstain extract of ethyl acetate Red Fruit (Pandanus Conoideus Lam.), 12 preparations produced a color close to color code 62B on the standard degradation of RHS color chart that is moderate purplish pink. Conclusions: From this preliminary study that examined the extract of ethyl acetate Red Fruit as a counterstain on Gram staining resulted in a color contrast between Gram + and Gram -bacteria though not as good as if using compressed/counterstain basic fuchsin.

Suggested Citation

  • Warta Dewi & Indrati & Inne Suherna Sasmita, 2017. "Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique," International Journal of Sciences, Office ijSciences, vol. 6(07), pages 28-32, July.
    • Handle: RePEc:adm:journl:v:6:y:2017:i:7:p:28-32
    DOI: 10.18483/ijSci.1357
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