Urogenital Mycoplasmas Detection in New Born Child’s with Respiratory Complications

Among premature infants, the bacterial colonization of the respiratory tract has been associated with the development of pneumonia, chronic lung disease, acute respiratory insufficiency, and even death. Genital mycoplasmas, particularly Mycoplasma hominis, M. genitalium, and Ureaplasma spp., have been associated with these pathological conditions, and also with neonatal pneumonia and respiratory dystress syndrome. The objective of this study was to determine the frequency of M. hominis, M. genitalium, U. parvum and U. urealyticum in newborn child’s, and to determine whether its presence is associated with the development of respiratory complications in these patients. Were analyzed 62 respiratory secretion samples (27 nasal swabs and 35 endotracheal tubes) obtained from 34 mechanical ventilated infants. Was realized the bacteriological culture for the isolation of Ureaplasma and Mycoplasma spp., and the PCR for the Mollicutes Class and Multiplex-PCR for the identification of U. urealyticum, U. parvum, M. hominis and M. genitalium species. The bacteriological culture was positive in one sample to mycoplasmas, while with the PCR for the Mollicutes Class was obtained 25 positive samples. From these, with the Multiplex-PCR were identified three positive samples to U. parvum, two positive to U. urealyticum and one positive to M. hominis. These six samples showed coinfection with other bacteria. Were identified mycoplasmas and ureaplasma species in new born ventilated child´s. Statistical association was not demonstrated between the mycoplasmas or ureaplasmas infection and respiratory complications in the infant.