IDEAS home Printed from https://ideas.repec.org/a/adm/journl/v5y2016i2p91-102.html
   My bibliography  Save this article

Effect of Lipopolysaccharide and 4-(Methylnitro-samino)-1- (3-pyridyl)-1-butanone on the Proliferation of Mouse Bone Marrow Stem Cells

Author

Listed:
  • Liu Xiaoping
  • Yu Zhongjie
  • Jiang Shasha
  • Wang Yi
  • Yang Tongxi
  • Wang Chunxue

Abstract

BMSCs were isolated, cultured and proliferated in vitro. The proliferation of mouse BMSCs treated with different concentrations of LPS and NNK were detected by MTT assay, the expression of NF-κB and CUGBP1 in the BMSCs were analyzed by Immunocytochemistry assay and Western Blot. MTT results shown that different concentrations of LPS and NNK could promote the proliferation of BMSCs and the promotion effect of LPS12.5μg/ml, NNK10μg/ml and LPS12.5μg/ml combined NNK 10μg/ml was much more significant than others. Immunocytochemistry and Western Blot revealed that the location and the expression level of NF-κB and CUGBP1 in BMSCs treated with LPS and NNK were changed with different incubation time. The highest expression of NF-κB was detected at the LPS48h, NNK24h and LPS combined NNK24h and significant difference can be seen in-group compared. The highest expression of CUGBP1 was LPS96h, NNK48h and LPS combined NNK24h and significant difference can be seen in-group compared. The irritation of LPS and NNK on the proliferation of mouse BMSCs may mediate by up-regulating the expression of NF-κB and CUGBP1.

Suggested Citation

  • Liu Xiaoping & Yu Zhongjie & Jiang Shasha & Wang Yi & Yang Tongxi & Wang Chunxue, 2016. "Effect of Lipopolysaccharide and 4-(Methylnitro-samino)-1- (3-pyridyl)-1-butanone on the Proliferation of Mouse Bone Marrow Stem Cells," International Journal of Sciences, Office ijSciences, vol. 5(02), pages 91-102, February.
    • Handle: RePEc:adm:journl:v:5:y:2016:i:2:p:91-102
    DOI: 10.18483/ijSci.925
    as

    Download full text from publisher

    File URL: https://www.ijsciences.com/pub/article/925
    Download Restriction: no
    File URL: https://www.ijsciences.com/pub/pdf/V5201602925.pdf
    Download Restriction: no
    File URL: https://libkey.io/10.18483/ijSci.925?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adm:journl:v:5:y:2016:i:2:p:91-102. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Staff ijSciences (email available below). General contact details of provider: .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.