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Development and Evaluation of Up-Converting Phosphor Technology-Based Lateral Flow Assay for Quantitative Detection of NT-proBNP in Blood

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Listed:
  • Xiaoli Yang
  • Liping Liu
  • Qingfang Hao
  • Deyong Zou
  • Xiaoli Zhang
  • Liping Zhang
  • Hongmei Li
  • Yong Qiao
  • Huansheng Zhao
  • Lei Zhou

Abstract

A newly assay, up-converting phosphor technology-based lateral flow (UPT-LF) assay, was developed for rapid and quantitative detection of N-terminal fragment of B-type natriuretic peptide precursor (NT-proBNP), one of the most important serum molecular maker of heat failure, in plasma samples as a point of care testing (POCT) method for diagnosis of acute heart failure. Human plasma from 197 patients with acute heart failure and 200 healthy controls was assessed using the UPT-LF assay, in a comparison with a Roche Elecsys assay. The limit of detection of the UPT-LF assay, with a coefficient of variation (CV) of less than 15%, was 116 ng/L, which is lower than the clinical diagnosis cutoff (150 ng/mL). The linear range was 50–35,000 ng/L. The CVs were less than 10% for both UPT-LF and Roche Elecsys assays for plasma samples under different storages, demonstrating the good stability and reproducibility. There are certain linear correlations between the results of UPT-LF and Roche Elecsys assay for EDTA-K2 and heparin-anticoagulated plasma, as well as for serum samples. For UPT-LF assay, there is a significant correlation between the values derived from analysis of EDTA-K2 and heparin-anticoagulated plasma samples (R = 0.995). No statistically significant difference was found between serum and plasma samples for UPT-LF assay. Our results demonstrate that NT-proBNP levels in healthy adults are elevated with age and had a relationship with sex, and with the age increase the NT-proBNP levels of females are significantly higher than those of males (p

Suggested Citation

  • Xiaoli Yang & Liping Liu & Qingfang Hao & Deyong Zou & Xiaoli Zhang & Liping Zhang & Hongmei Li & Yong Qiao & Huansheng Zhao & Lei Zhou, 2017. "Development and Evaluation of Up-Converting Phosphor Technology-Based Lateral Flow Assay for Quantitative Detection of NT-proBNP in Blood," PLOS ONE, Public Library of Science, vol. 12(2), pages 1-13, February.
    • Handle: RePEc:plo:pone00:0171376
    DOI: 10.1371/journal.pone.0171376
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