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Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC)

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  • Zachary F Phillips
  • Michael Chen
  • Laura Waller

Abstract

We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification—an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel.

Suggested Citation

  • Zachary F Phillips & Michael Chen & Laura Waller, 2017. "Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC)," PLOS ONE, Public Library of Science, vol. 12(2), pages 1-14, February.
    • Handle: RePEc:plo:pone00:0171228
    DOI: 10.1371/journal.pone.0171228
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