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An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity

Author

Listed:
  • Jie Huang

    (Nanjing Agricultural University)

  • Lianfeng Gu

    (Fujian Agriculture and Forestry University)

  • Ying Zhang

    (Nanjing Agricultural University)

  • Tingxiu Yan

    (Nanjing Agricultural University)

  • Guanghui Kong

    (Nanjing Agricultural University)

  • Liang Kong

    (Nanjing Agricultural University)

  • Baodian Guo

    (Nanjing Agricultural University
    Chinese Academy of Sciences)

  • Min Qiu

    (Nanjing Agricultural University)

  • Yang Wang

    (Nanjing Agricultural University)

  • Maofeng Jing

    (Nanjing Agricultural University)

  • Weiman Xing

    (Chinese Academy of Sciences)

  • Wenwu Ye

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Zhe Wu

    (South University of Science and Technology of China, Shenzhen)

  • Zhengguang Zhang

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Xiaobo Zheng

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Mark Gijzen

    (Agriculture and Agri-Food Canada)

  • Yuanchao Wang

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Suomeng Dong

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

Abstract

The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems.

Suggested Citation

  • Jie Huang & Lianfeng Gu & Ying Zhang & Tingxiu Yan & Guanghui Kong & Liang Kong & Baodian Guo & Min Qiu & Yang Wang & Maofeng Jing & Weiman Xing & Wenwu Ye & Zhe Wu & Zhengguang Zhang & Xiaobo Zheng &, 2017. "An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity," Nature Communications, Nature, vol. 8(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02233-5
    DOI: 10.1038/s41467-017-02233-5
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