IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-02222-8.html
   My bibliography  Save this article

Infection via mosquito bite alters Zika virus tissue tropism and replication kinetics in rhesus macaques

Author

Listed:
  • Dawn M. Dudley

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Christina M. Newman

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Joseph Lalli

    (University of Wisconsin-Madison School of Veterinary Medicine, 1656 Linden Dr.)

  • Laurel M. Stewart

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Michelle R. Koenig

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Andrea M. Weiler

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Matthew R. Semler

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Gabrielle L. Barry

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Katie R. Zarbock

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Mariel S. Mohns

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Meghan E. Breitbach

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.)

  • Nancy Schultz-Darken

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Eric Peterson

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Wendy Newton

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Emma L. Mohr

    (University of Wisconsin-Madison, University of Wisconsin Clinical Science Center, 600 Highland Ave)

  • Saverio Capuano III

    (University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Jorge E. Osorio

    (University of Wisconsin-Madison School of Veterinary Medicine, 1656 Linden Dr.)

  • Shelby L. O’Connor

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.
    University of Wisconsin-Madison, 1220 Capitol Ct.)

  • David H. O’Connor

    (University of Wisconsin-Madison School of Medicine and Public Health, 3170 UW Medical Foundation Centennial Building, 1685 Highland Ave.
    University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Thomas C. Friedrich

    (University of Wisconsin-Madison School of Veterinary Medicine, 1656 Linden Dr.
    University of Wisconsin-Madison, 1220 Capitol Ct.)

  • Matthew T. Aliota

    (University of Wisconsin-Madison School of Veterinary Medicine, 1656 Linden Dr.)

Abstract

Mouse and nonhuman primate models now serve as useful platforms to study Zika virus (ZIKV) pathogenesis, candidate therapies, and vaccines, but they rely on needle inoculation of virus: the effects of mosquito-borne infection on disease outcome have not been explored in these models. Here we show that infection via mosquito bite delays ZIKV replication to peak viral loads in rhesus macaques. Importantly, in mosquito-infected animals ZIKV tissue distribution was limited to hemolymphatic tissues, female reproductive tract tissues, kidney, and liver, potentially emulating key features of human ZIKV infections, most of which are characterized by mild or asymptomatic disease. Furthermore, deep sequencing analysis reveals that ZIKV populations in mosquito-infected monkeys show greater sequence heterogeneity and lower overall diversity than in needle-inoculated animals. This newly developed system will be valuable for studying ZIKV disease because it more closely mimics human infection by mosquito bite than needle-based inoculations.

Suggested Citation

  • Dawn M. Dudley & Christina M. Newman & Joseph Lalli & Laurel M. Stewart & Michelle R. Koenig & Andrea M. Weiler & Matthew R. Semler & Gabrielle L. Barry & Katie R. Zarbock & Mariel S. Mohns & Meghan E, 2017. "Infection via mosquito bite alters Zika virus tissue tropism and replication kinetics in rhesus macaques," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02222-8
    DOI: 10.1038/s41467-017-02222-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-02222-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-02222-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02222-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.