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Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution

Author

Listed:
  • Jae Young Kim

    (DGIST)

  • Eun Seok Seo

    (DGIST)

  • Hyunmin Kim

    (DGIST)

  • Ji-Won Park

    (Chungnam National University)

  • Dong-Kwon Lim

    (Korea University)

  • Dae Won Moon

    (DGIST)

Abstract

We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 µm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process.

Suggested Citation

  • Jae Young Kim & Eun Seok Seo & Hyunmin Kim & Ji-Won Park & Dong-Kwon Lim & Dae Won Moon, 2017. "Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02216-6
    DOI: 10.1038/s41467-017-02216-6
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