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A 1-phytase type III effector interferes with plant hormone signaling

Author

Listed:
  • Doreen Blüher

    (Martin-Luther University Halle-Wittenberg)

  • Debabrata Laha

    (University of Bonn
    Eberhard Karls University Tübingen)

  • Sabine Thieme

    (Martin-Luther University Halle-Wittenberg)

  • Alexandre Hofer

    (University of Zurich)

  • Lennart Eschen-Lippold

    (Leibniz Institute of Plant Biochemistry)

  • Antonia Masch

    (Martin-Luther University Halle-Wittenberg)

  • Gerd Balcke

    (Leibniz Institute of Plant Biochemistry)

  • Igor Pavlovic

    (Albert-Ludwigs University Freiburg)

  • Oliver Nagel

    (Martin-Luther University Halle-Wittenberg)

  • Antje Schonsky

    (Martin-Luther University Halle-Wittenberg)

  • Rahel Hinkelmann

    (Albert-Ludwigs University Freiburg)

  • Jakob Wörner

    (Albert-Ludwigs University Freiburg)

  • Nargis Parvin

    (Eberhard Karls University Tübingen)

  • Ralf Greiner

    (Max-Rubner-Institut, Federal Research Institute of Nutrition and Food)

  • Stefan Weber

    (Albert-Ludwigs University Freiburg)

  • Alain Tissier

    (Leibniz Institute of Plant Biochemistry)

  • Mike Schutkowski

    (Martin-Luther University Halle-Wittenberg)

  • Justin Lee

    (Leibniz Institute of Plant Biochemistry)

  • Henning Jessen

    (University of Zurich
    Albert-Ludwigs University Freiburg)

  • Gabriel Schaaf

    (University of Bonn
    Eberhard Karls University Tübingen)

  • Ulla Bonas

    (Martin-Luther University Halle-Wittenberg)

Abstract

Most Gram-negative phytopathogenic bacteria inject type III effector (T3E) proteins into plant cells to manipulate signaling pathways to the pathogen’s benefit. In resistant plants, specialized immune receptors recognize single T3Es or their biochemical activities, thus halting pathogen ingress. However, molecular function and mode of recognition for most T3Es remains elusive. Here, we show that the Xanthomonas T3E XopH possesses phytase activity, i.e., dephosphorylates phytate (myo-inositol-hexakisphosphate, InsP6), the major phosphate storage compound in plants, which is also involved in pathogen defense. A combination of biochemical approaches, including a new NMR-based method to discriminate inositol polyphosphate enantiomers, identifies XopH as a naturally occurring 1-phytase that dephosphorylates InsP6 at C1. Infection of Nicotiana benthamiana and pepper by Xanthomonas results in a XopH-dependent conversion of InsP6 to InsP5. 1-phytase activity is required for XopH-mediated immunity of plants carrying the Bs7 resistance gene, and for induction of jasmonate- and ethylene-responsive genes in N. benthamiana.

Suggested Citation

  • Doreen Blüher & Debabrata Laha & Sabine Thieme & Alexandre Hofer & Lennart Eschen-Lippold & Antonia Masch & Gerd Balcke & Igor Pavlovic & Oliver Nagel & Antje Schonsky & Rahel Hinkelmann & Jakob Wörne, 2017. "A 1-phytase type III effector interferes with plant hormone signaling," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02195-8
    DOI: 10.1038/s41467-017-02195-8
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