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Structural basis for the assembly of the Ragulator-Rag GTPase complex

Author

Listed:
  • Ryo Yonehara

    (Osaka University)

  • Shigeyuki Nada

    (Osaka University)

  • Tomokazu Nakai

    (Osaka University)

  • Masahiro Nakai

    (Osaka University)

  • Ayaka Kitamura

    (Osaka University)

  • Akira Ogawa

    (Osaka University)

  • Hirokazu Nakatsumi

    (Kyushu University)

  • Keiichi I. Nakayama

    (Kyushu University)

  • Songling Li

    (Osaka University)

  • Daron M. Standley

    (Osaka University)

  • Eiki Yamashita

    (Osaka University)

  • Atsushi Nakagawa

    (Osaka University)

  • Masato Okada

    (Osaka University)

Abstract

The mechanistic target of rapamycin complex 1 (mTORC1) plays a central role in regulating cell growth and metabolism by responding to cellular nutrient conditions. The activity of mTORC1 is controlled by Rag GTPases, which are anchored to lysosomes via Ragulator, a pentameric protein complex consisting of membrane-anchored p18/LAMTOR1 and two roadblock heterodimers. Here we report the crystal structure of Ragulator in complex with the roadblock domains of RagA-C, which helps to elucidate the molecular basis for the regulation of Rag GTPases. In the structure, p18 wraps around the three pairs of roadblock heterodimers to tandemly assemble them onto lysosomes. Cellular and in vitro analyses further demonstrate that p18 is required for Ragulator-Rag GTPase assembly and amino acid-dependent activation of mTORC1. These results establish p18 as a critical organizing scaffold for the Ragulator-Rag GTPase complex, which may provide a platform for nutrient sensing on lysosomes.

Suggested Citation

  • Ryo Yonehara & Shigeyuki Nada & Tomokazu Nakai & Masahiro Nakai & Ayaka Kitamura & Akira Ogawa & Hirokazu Nakatsumi & Keiichi I. Nakayama & Songling Li & Daron M. Standley & Eiki Yamashita & Atsushi N, 2017. "Structural basis for the assembly of the Ragulator-Rag GTPase complex," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01762-3
    DOI: 10.1038/s41467-017-01762-3
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