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Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Author

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  • Magalie Martineau

    (University of Bordeaux
    Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297)

  • Agila Somasundaram

    (US National Institutes of Health)

  • Jonathan B. Grimm

    (Howard Hughes Medical Institute)

  • Todd D. Gruber

    (Howard Hughes Medical Institute)

  • Daniel Choquet

    (University of Bordeaux
    Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297
    Université de Bordeaux, US 4 INSERM)

  • Justin W. Taraska

    (US National Institutes of Health)

  • Luke D. Lavis

    (Howard Hughes Medical Institute)

  • David Perrais

    (University of Bordeaux
    Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297)

Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

Suggested Citation

  • Magalie Martineau & Agila Somasundaram & Jonathan B. Grimm & Todd D. Gruber & Daniel Choquet & Justin W. Taraska & Luke D. Lavis & David Perrais, 2017. "Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01752-5
    DOI: 10.1038/s41467-017-01752-5
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