IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-01107-0.html
   My bibliography  Save this article

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration

Author

Listed:
  • Jessica Aijia Liu

    (The University of Hong Kong)

  • Yanxia Rao

    (The University of Hong Kong)

  • May Pui Lai Cheung

    (The University of Hong Kong)

  • Man-Ning Hui

    (The University of Hong Kong)

  • Ming-Hoi Wu

    (The University of Hong Kong)

  • Lo-Kong Chan

    (The University of Hong Kong)

  • Irene Oi-Lin Ng

    (The University of Hong Kong)

  • Ben Niu

    (The University of Hong Kong)

  • Kathryn S. E. Cheah

    (The University of Hong Kong)

  • Rakesh Sharma

    (The University of Hong Kong)

  • Louis Hodgson

    (Albert Einstein College of Medicine)

  • Martin Cheung

    (The University of Hong Kong)

Abstract

Following epithelial-mesenchymal transition, acquisition of avian trunk neural crest cell (NCC) polarity is prerequisite for directional delamination and migration, which in turn is essential for peripheral nervous system development. However, how this cell polarization is established and regulated remains unknown. Here we demonstrate that, using the RHOA biosensor in vivo and in vitro, the initiation of NCC polarization is accompanied by highly activated RHOA in the cytoplasm at the cell rear and its fluctuating activity at the front edge. This differential RHOA activity determines polarized NC morphology and motility, and is regulated by the asymmetrically localized RhoGAP Deleted in liver cancer (DLC1) in the cytoplasm at the cell front. Importantly, the association of DLC1 with NEDD9 is crucial for its asymmetric localization and differential RHOA activity. Moreover, NC specifiers, SOX9 and SOX10, regulate NEDD9 and DLC1 expression, respectively. These results present a SOX9/SOX10-NEDD9/DLC1-RHOA regulatory axis to govern NCC migratory polarization.

Suggested Citation

  • Jessica Aijia Liu & Yanxia Rao & May Pui Lai Cheung & Man-Ning Hui & Ming-Hoi Wu & Lo-Kong Chan & Irene Oi-Lin Ng & Ben Niu & Kathryn S. E. Cheah & Rakesh Sharma & Louis Hodgson & Martin Cheung, 2017. "Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration," Nature Communications, Nature, vol. 8(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01107-0
    DOI: 10.1038/s41467-017-01107-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-01107-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-01107-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01107-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.