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Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

Author

Listed:
  • Marco Orecchioni

    (Department of Chemistry and Pharmacy University of Sassari)

  • Davide Bedognetti

    (Sidra Medical and Research Center)

  • Leon Newman

    (Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester)

  • Claudia Fuoco

    (University of Rome Tor Vergata)

  • Filomena Spada

    (University of Rome Tor Vergata)

  • Wouter Hendrickx

    (Sidra Medical and Research Center)

  • Francesco M. Marincola

    (Sidra Medical and Research Center
    Abbvie Corporation)

  • Francesco Sgarrella

    (Department of Chemistry and Pharmacy University of Sassari)

  • Artur Filipe Rodrigues

    (Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester)

  • Cécilia Ménard-Moyon

    (Laboratoire d’Immunopathologie et Chimie Thérapeutique)

  • Gianni Cesareni

    (University of Rome Tor Vergata)

  • Kostas Kostarelos

    (Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester)

  • Alberto Bianco

    (Laboratoire d’Immunopathologie et Chimie Thérapeutique)

  • Lucia G Delogu

    (Department of Chemistry and Pharmacy University of Sassari
    Dresden University of Technology)

Abstract

Understanding the biomolecular interactions between graphene and human immune cells is a prerequisite for its utilization as a diagnostic or therapeutic tool. To characterize the complex interactions between graphene and immune cells, we propose an integrative analytical pipeline encompassing the evaluation of molecular and cellular parameters. Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO) and GO functionalized with amino groups (GONH2) on 15 immune cell populations, interrogating 30 markers at the single-cell level. Next, the integration of single-cell mass cytometry with genome-wide transcriptome analysis shows that the amine groups reduce the perturbations caused by GO on cell metabolism and increase biocompatibility. Moreover, GONH2 polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response. This study describes an innovative approach for the analysis of the effects of nanomaterials on distinct immune cells, laying the foundation for the incorporation of single-cell mass cytometry on the experimental pipeline.

Suggested Citation

  • Marco Orecchioni & Davide Bedognetti & Leon Newman & Claudia Fuoco & Filomena Spada & Wouter Hendrickx & Francesco M. Marincola & Francesco Sgarrella & Artur Filipe Rodrigues & Cécilia Ménard-Moyon & , 2017. "Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells," Nature Communications, Nature, vol. 8(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01015-3
    DOI: 10.1038/s41467-017-01015-3
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