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Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium

Author

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  • Young Jun An

    (Korea Institute of Ocean Science and Technology)

  • Sara E. Rowland

    (University of Maryland
    Institute of Marine and Environmental Technology)

  • Jung-Hyun Na

    (Ewha Womans University)

  • Dario Spigolon

    (University of Maryland
    Institute of Marine and Environmental Technology)

  • Seung Kon Hong

    (Ewha Womans University)

  • Yeo Joon Yoon

    (Ewha Womans University)

  • Jung-Hyun Lee

    (Korea Institute of Ocean Science and Technology)

  • Frank T. Robb

    (University of Maryland
    Institute of Marine and Environmental Technology)

  • Sun-Shin Cha

    (Ewha Womans University)

Abstract

The chaperonins (CPNs) are megadalton sized hollow complexes with two cavities that open and close to encapsulate non-native proteins. CPNs are assigned to two sequence-related groups that have distinct allosteric mechanisms. In Group I CPNs a detachable co-chaperone, GroES, closes the chambers whereas in Group II a built-in lid closes the chambers. Group I CPNs have a bacterial ancestry, whereas Group II CPNs are archaeal in origin. Here we describe open and closed crystal structures representing a new phylogenetic branch of CPNs. These Group III CPNs are divergent in sequence and structure from extant CPNs, but are closed by a built-in lid like Group II CPNs. A nucleotide-sensing loop, present in both Group I and Group II CPNs, is notably absent. We identified inter-ring pivot joints that articulate during ring closure. These Group III CPNs likely represent a relic from the ancestral CPN that formed distinct bacterial and archaeal branches.

Suggested Citation

  • Young Jun An & Sara E. Rowland & Jung-Hyun Na & Dario Spigolon & Seung Kon Hong & Yeo Joon Yoon & Jung-Hyun Lee & Frank T. Robb & Sun-Shin Cha, 2017. "Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00980-z
    DOI: 10.1038/s41467-017-00980-z
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