IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-00863-3.html
   My bibliography  Save this article

Monomeric ephrinB2 binding induces allosteric changes in Nipah virus G that precede its full activation

Author

Listed:
  • Joyce J. W. Wong

    (Stanford University School of Medicine)

  • Tracy A. Young

    (Biodesy, Inc.)

  • Jiayan Zhang

    (University of California Los Angeles
    University of California Los Angeles
    University of California Los Angeles)

  • Shiheng Liu

    (University of California Los Angeles
    University of California Los Angeles)

  • George P. Leser

    (Northwestern University
    Northwestern University)

  • Elizabeth A. Komives

    (University of California San Diego)

  • Robert A. Lamb

    (Northwestern University
    Northwestern University)

  • Z. Hong Zhou

    (University of California Los Angeles
    University of California Los Angeles)

  • Joshua Salafsky

    (Biodesy, Inc.)

  • Theodore S. Jardetzky

    (Stanford University School of Medicine)

Abstract

Nipah virus is an emergent paramyxovirus that causes deadly encephalitis and respiratory infections in humans. Two glycoproteins coordinate the infection of host cells, an attachment protein (G), which binds to cell surface receptors, and a fusion (F) protein, which carries out the process of virus-cell membrane fusion. The G protein binds to ephrin B2/3 receptors, inducing G conformational changes that trigger F protein refolding. Using an optical approach based on second harmonic generation, we show that monomeric and dimeric receptors activate distinct conformational changes in G. The monomeric receptor-induced changes are not detected by conformation-sensitive monoclonal antibodies or through electron microscopy analysis of G:ephrinB2 complexes. However, hydrogen/deuterium exchange experiments confirm the second harmonic generation observations and reveal allosteric changes in the G receptor binding and F-activating stalk domains, providing insights into the pathway of receptor-activated virus entry.

Suggested Citation

  • Joyce J. W. Wong & Tracy A. Young & Jiayan Zhang & Shiheng Liu & George P. Leser & Elizabeth A. Komives & Robert A. Lamb & Z. Hong Zhou & Joshua Salafsky & Theodore S. Jardetzky, 2017. "Monomeric ephrinB2 binding induces allosteric changes in Nipah virus G that precede its full activation," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00863-3
    DOI: 10.1038/s41467-017-00863-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-00863-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-00863-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00863-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.