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Endoderm Jagged induces liver and pancreas duct lineage in zebrafish

Author

Listed:
  • Danhua Zhang

    (Sanford Burnham Prebys Medical Discovery Institute
    Science)

  • Keith P. Gates

    (Sanford Burnham Prebys Medical Discovery Institute)

  • Lindsey Barske

    (Keck School of Medicine of University of Southern California)

  • Guangliang Wang

    (Johns Hopkins University School of Medicine)

  • Joseph J. Lancman

    (Sanford Burnham Prebys Medical Discovery Institute)

  • Xin-Xin I. Zeng

    (Sanford Burnham Prebys Medical Discovery Institute)

  • Megan Groff

    (Keck School of Medicine of University of Southern California)

  • Kasper Wang

    (Keck School of Medicine of University of Southern California)

  • Michael J. Parsons

    (Johns Hopkins University School of Medicine)

  • J. Gage Crump

    (Keck School of Medicine of University of Southern California)

  • P. Duc Si Dong

    (Sanford Burnham Prebys Medical Discovery Institute
    Science)

Abstract

Liver duct paucity is characteristic of children born with Alagille Syndrome (ALGS), a disease associated with JAGGED1 mutations. Here, we report that zebrafish embryos with compound homozygous mutations in two Notch ligand genes, jagged1b (jag1b) and jagged2b (jag2b) exhibit a complete loss of canonical Notch activity and duct cells within the liver and exocrine pancreas, whereas hepatocyte and acinar pancreas development is not affected. Further, animal chimera studies demonstrate that wild-type endoderm cells within the liver and pancreas can rescue Notch activity and duct lineage specification in adjacent cells lacking jag1b and jag2b expression. We conclude that these two Notch ligands are directly and solely responsible for all duct lineage specification in these organs in zebrafish. Our study uncovers genes required for lineage specification of the intrahepatopancreatic duct cells, challenges the role of duct cells as progenitors, and suggests a genetic mechanism for ALGS ductal paucity.

Suggested Citation

  • Danhua Zhang & Keith P. Gates & Lindsey Barske & Guangliang Wang & Joseph J. Lancman & Xin-Xin I. Zeng & Megan Groff & Kasper Wang & Michael J. Parsons & J. Gage Crump & P. Duc Si Dong, 2017. "Endoderm Jagged induces liver and pancreas duct lineage in zebrafish," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00666-6
    DOI: 10.1038/s41467-017-00666-6
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