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SRpHi ratiometric pH biosensors for super-resolution microscopy

Author

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  • Douglas S. Richardson

    (Max Planck Institute for Biophysical Chemistry
    Harvard Center for Biological Imaging, Harvard University)

  • Carola Gregor

    (Max Planck Institute for Biophysical Chemistry)

  • Franziska R. Winter

    (Max Planck Institute for Biophysical Chemistry)

  • Nicolai T. Urban

    (Max Planck Institute for Biophysical Chemistry)

  • Steffen J. Sahl

    (Max Planck Institute for Biophysical Chemistry)

  • Katrin I. Willig

    (Max Planck Institute for Biophysical Chemistry
    University Medical Center Göttingen)

  • Stefan W. Hell

    (Max Planck Institute for Biophysical Chemistry
    Max Planck Institute for Medical Research)

Abstract

Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.

Suggested Citation

  • Douglas S. Richardson & Carola Gregor & Franziska R. Winter & Nicolai T. Urban & Steffen J. Sahl & Katrin I. Willig & Stefan W. Hell, 2017. "SRpHi ratiometric pH biosensors for super-resolution microscopy," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00606-4
    DOI: 10.1038/s41467-017-00606-4
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