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A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation

Author

Listed:
  • Vicard Du

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Nathalie Luciani

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Sophie Richard

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Gaëtan Mary

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Cyprien Gay

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • François Mazuel

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Myriam Reffay

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

  • Philippe Menasché

    (Université Paris Descartes)

  • Onnik Agbulut

    (Biological Adaptation and Ageing)

  • Claire Wilhelm

    (Laboratoire Matière et Systèmes Complexes (MSC), UMR 7057, CNRS and Université Paris Diderot)

Abstract

The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body. This magnetic approach to embryoid body formation has no discernible impact on ESC differentiation pathways, as compared to the hanging drop method. It is also the base of the final magnetic device, composed of opposing magnetic attractors in order to form embryoid bodies in situ, then stretch them, and mechanically stimulate them at will. These stretched and cyclic purely mechanical stimulations were sufficient to drive ESCs differentiation towards the mesodermal cardiac pathway.

Suggested Citation

  • Vicard Du & Nathalie Luciani & Sophie Richard & Gaëtan Mary & Cyprien Gay & François Mazuel & Myriam Reffay & Philippe Menasché & Onnik Agbulut & Claire Wilhelm, 2017. "A 3D magnetic tissue stretcher for remote mechanical control of embryonic stem cell differentiation," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00543-2
    DOI: 10.1038/s41467-017-00543-2
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