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Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes

Author

Listed:
  • Hoang Long Pham

    (National University of Singapore
    National University of Singapore)

  • Adison Wong

    (National University of Singapore
    National University of Singapore
    Singapore Institute of Technology)

  • Niying Chua

    (National University of Singapore
    National University of Singapore)

  • Wei Suong Teo

    (National University of Singapore
    National University of Singapore
    Ngee Ann Polytechnic)

  • Wen Shan Yew

    (National University of Singapore
    National University of Singapore)

  • Matthew Wook Chang

    (National University of Singapore
    National University of Singapore)

Abstract

Environmental pH is a fundamental signal continuously directing the metabolism and behavior of living cells. Programming the precise cellular response toward environmental pH is, therefore, crucial for engineering cells for increasingly sophisticated functions. Herein, we engineer a set of riboswitch-based pH-sensing genetic devices to enable the control of gene expression according to differential environmental pH. We next develop a digital pH-sensing system to utilize the analogue-sensing behavior of these devices for high-resolution recording of host cell exposure to discrete external pH levels. The application of this digital pH-sensing system is demonstrated in a genetic program that autonomously regulated the evolutionary engineering of host cells for improved tolerance to a broad spectrum of organic acids, a valuable phenotype for metabolic engineering and bioremediation applications.

Suggested Citation

  • Hoang Long Pham & Adison Wong & Niying Chua & Wei Suong Teo & Wen Shan Yew & Matthew Wook Chang, 2017. "Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00511-w
    DOI: 10.1038/s41467-017-00511-w
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