IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_s41467-017-00291-3.html
   My bibliography  Save this article

Single-cell analyses identify bioengineered niches for enhanced maintenance of hematopoietic stem cells

Author

Listed:
  • Aline Roch

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Sonja Giger

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Mukul Girotra

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Vasco Campos

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Nicola Vannini

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Olaia Naveiras

    (Ecole Polytechnique Fédérale de Lausanne (EPFL)
    Centre Hospitaler Universitaire Vaudois (CHUV))

  • Samy Gobaa

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Matthias P. Lutolf

    (Ecole Polytechnique Fédérale de Lausanne (EPFL)
    School of Basic Sciences, EPFL)

Abstract

The in vitro expansion of long-term hematopoietic stem cells (HSCs) remains a substantial challenge, largely because of our limited understanding of the mechanisms that control HSC fate choices. Using single-cell multigene expression analysis and time-lapse microscopy, here we define gene expression signatures and cell cycle hallmarks of murine HSCs and the earliest multipotent progenitors (MPPs), and analyze systematically single HSC fate choices in culture. Our analysis revealed twelve differentially expressed genes marking the quiescent HSC state, including four genes encoding cell–cell interaction signals in the niche. Under basal culture conditions, most HSCs rapidly commit to become early MPPs. In contrast, when we present ligands of the identified niche components such as JamC or Esam within artificial niches, HSC cycling is reduced and long-term multipotency in vivo is maintained. Our approach to bioengineer artificial niches should be useful in other stem cell systems.

Suggested Citation

  • Aline Roch & Sonja Giger & Mukul Girotra & Vasco Campos & Nicola Vannini & Olaia Naveiras & Samy Gobaa & Matthias P. Lutolf, 2017. "Single-cell analyses identify bioengineered niches for enhanced maintenance of hematopoietic stem cells," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00291-3
    DOI: 10.1038/s41467-017-00291-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-00291-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-00291-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00291-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.