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Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine

Author

Listed:
  • Johanna Marsian

    (Norwich Research Park)

  • Helen Fox

    (The National Institute for Biological Standards and Control)

  • Mohammad W. Bahar

    (University of Oxford, The Henry Wellcome Building for Genomic Medicine)

  • Abhay Kotecha

    (University of Oxford, The Henry Wellcome Building for Genomic Medicine)

  • Elizabeth E. Fry

    (University of Oxford, The Henry Wellcome Building for Genomic Medicine)

  • David I. Stuart

    (University of Oxford, The Henry Wellcome Building for Genomic Medicine
    Diamond Light Source, Harwell Science and Innovation Campus)

  • Andrew J. Macadam

    (The National Institute for Biological Standards and Control)

  • David J. Rowlands

    (University of Leeds)

  • George P. Lomonossoff

    (Norwich Research Park)

Abstract

Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced stabilized virus-like particle (sVLP)-based vaccine with D antigenicity, without the drawbacks of current vaccines, will be a major step towards the final eradication of poliovirus. Such a sVLP would retain the native antigenic conformation and the repetitive structure of the original virus particle, but lack infectious genomic material. In this study, we report the production of synthetically stabilized PV VLPs in plants. Mice carrying the gene for the human PV receptor are protected from wild-type PV when immunized with the plant-made PV sVLPs. Structural analysis of the stabilized mutant at 3.6 Å resolution by cryo-electron microscopy and single-particle reconstruction reveals a structure almost indistinguishable from wild-type PV3.

Suggested Citation

  • Johanna Marsian & Helen Fox & Mohammad W. Bahar & Abhay Kotecha & Elizabeth E. Fry & David I. Stuart & Andrew J. Macadam & David J. Rowlands & George P. Lomonossoff, 2017. "Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00090-w
    DOI: 10.1038/s41467-017-00090-w
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