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Quantitative real-time imaging of glutathione

Author

Listed:
  • Xiqian Jiang

    (Baylor College of Medicine)

  • Jianwei Chen

    (Baylor College of Medicine)

  • Aleksandar Bajić

    (Baylor College of Medicine
    Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital)

  • Chengwei Zhang

    (Baylor College of Medicine)

  • Xianzhou Song

    (Baylor College of Medicine)

  • Shaina L. Carroll

    (Baylor College of Medicine
    Rice University)

  • Zhao-Lin Cai

    (Baylor College of Medicine
    The Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital)

  • Meiling Tang

    (Baylor College of Medicine)

  • Mingshan Xue

    (Baylor College of Medicine
    The Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital
    Baylor College of Medicine)

  • Ninghui Cheng

    (Baylor College of Medicine
    USDA/ARS Children Nutrition Research Center, Baylor College of Medicine)

  • Christian P. Schaaf

    (Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital
    Baylor College of Medicine)

  • Feng Li

    (Baylor College of Medicine
    Center for Drug Discovery, Baylor College of Medicine)

  • Kevin R. MacKenzie

    (Center for Drug Discovery, Baylor College of Medicine
    Baylor College of Medicine)

  • Allan Chris M. Ferreon

    (Baylor College of Medicine)

  • Fan Xia

    (Baylor College of Medicine)

  • Meng C. Wang

    (Baylor College of Medicine
    Huffington Center on Aging, Baylor College of Medicine)

  • Mirjana Maletić-Savatić

    (Baylor College of Medicine
    Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital
    Center for Drug Discovery, Baylor College of Medicine)

  • Jin Wang

    (Baylor College of Medicine
    Baylor College of Medicine
    Center for Drug Discovery, Baylor College of Medicine)

Abstract

Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe—designated as RealThiol (RT)—that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

Suggested Citation

  • Xiqian Jiang & Jianwei Chen & Aleksandar Bajić & Chengwei Zhang & Xianzhou Song & Shaina L. Carroll & Zhao-Lin Cai & Meiling Tang & Mingshan Xue & Ninghui Cheng & Christian P. Schaaf & Feng Li & Kevin, 2017. "Quantitative real-time imaging of glutathione," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms16087
    DOI: 10.1038/ncomms16087
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