IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14751.html
   My bibliography  Save this article

An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells

Author

Listed:
  • Xiaofeng Huang

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Min Zheng

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Pui Wang

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Bobo Wing-Yee Mok

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Siwen Liu

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Siu-Ying Lau

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong)

  • Pin Chen

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Yen-Chin Liu

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Honglian Liu

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong)

  • Yixin Chen

    (National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University)

  • Wenjun Song

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    College of Life Science and Technology, Jinan University)

  • Kwok-Yung Yuen

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    College of Life Science and Technology, Jinan University)

  • Honglin Chen

    (State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong
    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong
    National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University)

Abstract

Influenza virus utilizes host splicing machinery to process viral mRNAs expressed from both M and NS segments. Through genetic analysis and functional characterization, we here show that the NS segment of H7N9 virus contains a unique G540A substitution, located within a previously undefined exonic splicing enhancer (ESE) motif present in the NEP mRNA of influenza A viruses. G540A supports virus replication in mammalian cells while retaining replication ability in avian cells. Host splicing regulator, SF2, interacts with this ESE to regulate splicing of NEP/NS1 mRNA and G540A substitution affects SF2–ESE interaction. The NS1 protein directly interacts with SF2 in the nucleus and modulates splicing of NS mRNAs during virus replication. We demonstrate that splicing of NEP/NS1 mRNA is regulated through a cis NEP-ESE motif and suggest a unique NEP-ESE may contribute to provide H7N9 virus with the ability to both circulate efficiently in avian hosts and replicate in mammalian cells.

Suggested Citation

  • Xiaofeng Huang & Min Zheng & Pui Wang & Bobo Wing-Yee Mok & Siwen Liu & Siu-Ying Lau & Pin Chen & Yen-Chin Liu & Honglian Liu & Yixin Chen & Wenjun Song & Kwok-Yung Yuen & Honglin Chen, 2017. "An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells," Nature Communications, Nature, vol. 8(1), pages 1-15, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14751
    DOI: 10.1038/ncomms14751
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14751
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14751?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14751. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.