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A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

Author

Listed:
  • Shuo Xiao

    (Feinberg School of Medicine, Northwestern University)

  • Jonathan R. Coppeta

    (The Charles Stark Draper Laboratory)

  • Hunter B. Rogers

    (Feinberg School of Medicine, Northwestern University)

  • Brett C. Isenberg

    (The Charles Stark Draper Laboratory)

  • Jie Zhu

    (Feinberg School of Medicine, Northwestern University)

  • Susan A. Olalekan

    (Feinberg School of Medicine, Northwestern University)

  • Kelly E. McKinnon

    (Feinberg School of Medicine, Northwestern University)

  • Danijela Dokic

    (Feinberg School of Medicine, Northwestern University)

  • Alexandra S. Rashedi

    (Feinberg School of Medicine, Northwestern University)

  • Daniel J. Haisenleder

    (Ligand Assay and Analysis Core, Center for Research in Reproduction, University of Virginia)

  • Saurabh S. Malpani

    (Feinberg School of Medicine, Northwestern University)

  • Chanel A. Arnold-Murray

    (Feinberg School of Medicine, Northwestern University)

  • Kuanwei Chen

    (Feinberg School of Medicine, Northwestern University)

  • Mingyang Jiang

    (Feinberg School of Medicine, Northwestern University)

  • Lu Bai

    (Feinberg School of Medicine, Northwestern University)

  • Catherine T. Nguyen

    (Feinberg School of Medicine, Northwestern University)

  • Jiyang Zhang

    (Feinberg School of Medicine, Northwestern University)

  • Monica M. Laronda

    (Feinberg School of Medicine, Northwestern University)

  • Thomas J. Hope

    (Feinberg School of Medicine, Northwestern University)

  • Kruti P. Maniar

    (Feinberg School of Medicine, Northwestern University)

  • Mary Ellen Pavone

    (Feinberg School of Medicine, Northwestern University)

  • Michael J. Avram

    (Feinberg School of Medicine, Northwestern University)

  • Elizabeth C. Sefton

    (Feinberg School of Medicine, Northwestern University)

  • Spiro Getsios

    (Feinberg School of Medicine, Northwestern University)

  • Joanna E. Burdette

    (University of Illinois at Chicago)

  • J. Julie Kim

    (Feinberg School of Medicine, Northwestern University)

  • Jeffrey T. Borenstein

    (The Charles Stark Draper Laboratory)

  • Teresa K. Woodruff

    (Feinberg School of Medicine, Northwestern University)

Abstract

The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ–organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

Suggested Citation

  • Shuo Xiao & Jonathan R. Coppeta & Hunter B. Rogers & Brett C. Isenberg & Jie Zhu & Susan A. Olalekan & Kelly E. McKinnon & Danijela Dokic & Alexandra S. Rashedi & Daniel J. Haisenleder & Saurabh S. Ma, 2017. "A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle," Nature Communications, Nature, vol. 8(1), pages 1-13, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14584
    DOI: 10.1038/ncomms14584
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