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Expanding the genetic code of Mus musculus

Author

Listed:
  • Songmi Han

    (Ajou University School of Medicine)

  • Aerin Yang

    (Korea Advanced Institute of Science and Technology)

  • Soonjang Lee

    (Ajou University School of Medicine)

  • Han-Woong Lee

    (Yonsei Laboratory Animal Research Center, Yonsei University)

  • Chan Bae Park

    (Ajou University School of Medicine)

  • Hee-Sung Park

    (Korea Advanced Institute of Science and Technology)

Abstract

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

Suggested Citation

  • Songmi Han & Aerin Yang & Soonjang Lee & Han-Woong Lee & Chan Bae Park & Hee-Sung Park, 2017. "Expanding the genetic code of Mus musculus," Nature Communications, Nature, vol. 8(1), pages 1-7, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14568
    DOI: 10.1038/ncomms14568
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