IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14533.html
   My bibliography  Save this article

Clonal reversal of ageing-associated stem cell lineage bias via a pluripotent intermediate

Author

Listed:
  • Martin Wahlestedt

    (Lund University, Medical Faculty, Institution for Laboratory Medicine)

  • Eva Erlandsson

    (Lund University, Medical Faculty, Institution for Laboratory Medicine)

  • Trine Kristiansen

    (Lund University, Medical Faculty, Institution for Laboratory Medicine)

  • Rong Lu

    (Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California)

  • Cord Brakebusch

    (Biotech Research and Innovation Centre, Biomedical Institute, University of Copenhagen
    Core Facility for Transgenic Mice, The Panum Institute)

  • Irving L. Weissman

    (Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford University)

  • Joan Yuan

    (Lund University, Medical Faculty, Institution for Laboratory Medicine
    StemTherapy, Lund University)

  • Javier Martin-Gonzalez

    (Core Facility for Transgenic Mice, The Panum Institute)

  • David Bryder

    (Lund University, Medical Faculty, Institution for Laboratory Medicine
    StemTherapy, Lund University)

Abstract

Ageing associates with significant alterations in somatic/adult stem cells and therapies to counteract these might have profound benefits for health. In the blood, haematopoietic stem cell (HSC) ageing is linked to several functional shortcomings. However, besides the recent realization that individual HSCs might be preset differentially already from young age, HSCs might also age asynchronously. Evaluating the prospects for HSC rejuvenation therefore ultimately requires approaching those HSCs that are functionally affected by age. Here we combine genetic barcoding of aged murine HSCs with the generation of induced pluripotent stem (iPS) cells. This allows us to specifically focus on aged HSCs presenting with a pronounced lineage skewing, a hallmark of HSC ageing. Functional and molecular evaluations reveal haematopoiesis from these iPS clones to be indistinguishable from that associating with young mice. Our data thereby provide direct support to the notion that several key functional attributes of HSC ageing can be reversed.

Suggested Citation

  • Martin Wahlestedt & Eva Erlandsson & Trine Kristiansen & Rong Lu & Cord Brakebusch & Irving L. Weissman & Joan Yuan & Javier Martin-Gonzalez & David Bryder, 2017. "Clonal reversal of ageing-associated stem cell lineage bias via a pluripotent intermediate," Nature Communications, Nature, vol. 8(1), pages 1-8, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14533
    DOI: 10.1038/ncomms14533
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14533
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14533?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14533. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.