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Recurring RNA structural motifs underlie the mechanics of L1 stalk movement

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  • Srividya Mohan

    (Cell and Developmental Biology, University of California at Santa Cruz)

  • Harry F Noller

    (Cell and Developmental Biology, University of California at Santa Cruz)

Abstract

The L1 stalk of the large ribosomal subunit undergoes large-scale movements coupled to the translocation of deacylated tRNA during protein synthesis. We use quantitative comparative structural analysis to localize the origins of L1 stalk movement and to understand its dynamic interactions with tRNA and other structural elements of the ribosome. Besides its stacking interactions with the tRNA elbow, stalk movement is directly linked to intersubunit rotation, rotation of the 30S head domain and contact of the acceptor arm of deacylated tRNA with helix 68 of 23S rRNA. Movement originates from pivoting at stacked non-canonical base pairs in a Family A three-way junction and bending in an internal G-U-rich zone. Use of these same motifs as hinge points to enable such dynamic events as rotation of the 30S subunit head domain and in flexing of the anticodon arm of tRNA suggests that they represent general strategies for movement of functional RNAs.

Suggested Citation

  • Srividya Mohan & Harry F Noller, 2017. "Recurring RNA structural motifs underlie the mechanics of L1 stalk movement," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14285
    DOI: 10.1038/ncomms14285
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