IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14246.html
   My bibliography  Save this article

A non-canonical mismatch repair pathway in prokaryotes

Author

Listed:
  • A. Castañeda-García

    (Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N
    Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • A. I. Prieto

    (Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N)

  • J. Rodríguez-Beltrán

    (Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N)

  • N. Alonso

    (Centro Nacional de Biotecnología-CSIC. C/ Darwin 3)

  • D. Cantillon

    (Brighton and Sussex Medical School, University of Sussex)

  • C. Costas

    (Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N)

  • L. Pérez-Lago

    (Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón and Instituto de Investigación Sanitaria Gregorio Marañón. Dr. Esquerdo 46)

  • E. D. Zegeye

    (Oslo University Hospital, Rikshospitalet, Oslo, University of Oslo)

  • M. Herranz

    (Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón and Instituto de Investigación Sanitaria Gregorio Marañón. Dr. Esquerdo 46)

  • P. Plociński

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
    Present address: Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warszawa, Poland)

  • T. Tonjum

    (Oslo University Hospital, Rikshospitalet, Oslo, University of Oslo)

  • D. García de Viedma

    (Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón and Instituto de Investigación Sanitaria Gregorio Marañón. Dr. Esquerdo 46)

  • M. Paget

    (School of Life Sciences, University of Sussex)

  • S. J. Waddell

    (Brighton and Sussex Medical School, University of Sussex)

  • A. M. Rojas

    (Computational Biology and Bioinformatics, Instituto de Biomedicina de Sevilla (IBIS)-CSIC. Avda. Manuel Siurot S/N)

  • A. J. Doherty

    (Genome Damage and Stability Centre, School of Life Sciences, University of Sussex)

  • J. Blázquez

    (Stress and Bacterial Evolution Group, Instituto de Biomedicina de Sevilla. Avda. Manuel Siurot S/N
    Centro Nacional de Biotecnología-CSIC. C/ Darwin 3
    Unit of Infectious Diseases, Microbiology, and Preventive Medicine. University Hospital Virgen del Rocio, Avda. Manuel Siurot S/N)

Abstract

Mismatch repair (MMR) is a near ubiquitous pathway, essential for the maintenance of genome stability. Members of the MutS and MutL protein families perform key steps in mismatch correction. Despite the major importance of this repair pathway, MutS–MutL are absent in almost all Actinobacteria and many Archaea. However, these organisms exhibit rates and spectra of spontaneous mutations similar to MMR-bearing species, suggesting the existence of an alternative to the canonical MutS–MutL-based MMR. Here we report that Mycobacterium smegmatis NucS/EndoMS, a putative endonuclease with no structural homology to known MMR factors, is required for mutation avoidance and anti-recombination, hallmarks of the canonical MMR. Furthermore, phenotypic analysis of naturally occurring polymorphic NucS in a M. smegmatis surrogate model, suggests the existence of M. tuberculosis mutator strains. The phylogenetic analysis of NucS indicates a complex evolutionary process leading to a disperse distribution pattern in prokaryotes. Together, these findings indicate that distinct pathways for MMR have evolved at least twice in nature.

Suggested Citation

  • A. Castañeda-García & A. I. Prieto & J. Rodríguez-Beltrán & N. Alonso & D. Cantillon & C. Costas & L. Pérez-Lago & E. D. Zegeye & M. Herranz & P. Plociński & T. Tonjum & D. García de Viedma & M. Paget, 2017. "A non-canonical mismatch repair pathway in prokaryotes," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14246
    DOI: 10.1038/ncomms14246
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14246
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14246?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14246. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.