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Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy

Author

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  • Malte Kühnemund

    (Science for Life Laboratory, genetics and pathology, Uppsala University
    Science for Life Laboratory, Stockholm University, Sweden)

  • Qingshan Wei

    (University of California, Los Angeles
    University of California, Los Angeles
    California NanoSystems Institute (CNSI), University of California, Los Angeles)

  • Evangelia Darai

    (Science for Life Laboratory, Stockholm University, Sweden)

  • Yingjie Wang

    (University of California, Los Angeles)

  • Iván Hernández-Neuta

    (Science for Life Laboratory, Stockholm University, Sweden)

  • Zhao Yang

    (University of California, Los Angeles)

  • Derek Tseng

    (University of California, Los Angeles)

  • Annika Ahlford

    (Science for Life Laboratory, Stockholm University, Sweden
    Devyser AB)

  • Lucy Mathot

    (Science for Life Laboratory, genetics and pathology, Uppsala University)

  • Tobias Sjöblom

    (Science for Life Laboratory, genetics and pathology, Uppsala University)

  • Aydogan Ozcan

    (University of California, Los Angeles
    University of California, Los Angeles
    California NanoSystems Institute (CNSI), University of California, Los Angeles)

  • Mats Nilsson

    (Science for Life Laboratory, genetics and pathology, Uppsala University
    Science for Life Laboratory, Stockholm University, Sweden)

Abstract

Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.

Suggested Citation

  • Malte Kühnemund & Qingshan Wei & Evangelia Darai & Yingjie Wang & Iván Hernández-Neuta & Zhao Yang & Derek Tseng & Annika Ahlford & Lucy Mathot & Tobias Sjöblom & Aydogan Ozcan & Mats Nilsson, 2017. "Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy," Nature Communications, Nature, vol. 8(1), pages 1-8, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms13913
    DOI: 10.1038/ncomms13913
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